Introduction Copy the liquid sample to the agar solid plate Advantage It is important to set the appropriate vaccination height during library replication. For sample source plates, the inoculation height is set to the point where the spotting needle is in sufficient contact with the liquid E. coli sample in the 96-well plate. For the target solid plate, the inoculation height is set to the spotted needle just in contact with OmniTray's LB agar solid plate (Figure 2). After the library copying operation is completed, the spotted position traces of the inoculated samples can be clearly seen on the agar plates (Figure 3A). After 2 days of incubation at 37 degrees, it was observed that the E. coli samples grew well on the inoculated OmniTray LB agar plates (Figure 3B). Agar to agar solid plate replication in conclusion Ffp2 Kn95 Face Mask,Ppf2 Protective Cup Mask,Kn95 Protective Cup Mask,Ppf2 Protective Fold Flat Mask Jiangmen anjian biotechnology co. LTD , https://www.jmanjianmask.com
Library replication between the same microplates of conventional liquid samples can be performed using the QPix400 system (eg, copying from 96 to 96 well plates, copying from 384 to 384 well plates). However, copying library samples from agar plates to agar plates for solid-to-solid replication is also a useful tool, such as screening for gene resistance or yeast two-hybrid screening. This article describes how to perform a library copy operation on a QPix400 system (Figure 1), such as copying a sample from a liquid to a solid, from a solid to a solid.
The E. coli sample was cultured overnight at 37 degrees in 10 ml of LB medium containing Amp. Then, take 100 ul of E. coli sample to a standard 96-well plate. In order to complete this experiment, the QPix420 system's copy rearrangement software module and the Gridding head were used. The sample plate contains the cultured liquid sample, and the target microplate is an empty agar plate for inoculating the sample in the sample plate.
Figure 1. QPix420 System Software Interface: The red-labeled library copy function is located under the QPix420 System Copy Function Module. 
Figure 2. Library replication inoculation height setting: The inoculation height needs to be adjusted according to the type of microplate and the thickness of the agar. 
Figure 3. Photograph of the OmniTray plate after completion of the replication of the liquid E. coli sample from the standard 96-well plate to the OmniTray LB agar solid plate: A) After the inoculation is completed, the sample position of the 96 inoculation needle can be clearly seen on the OmniTray plate. B) After the same piece of OmniTray plate was cultured at 37 degrees for 2 days, the E.coli clone after obvious growth could be seen in each inoculated sample position, indicating that the library replication operation can successfully take the liquid E.coli sample from the 96-well sample. Transfer the plate to OmniTray LB agar solid plate
To replicate samples from OmniTray LB agar plates to OmniTray LB agar plates, the library replication module and spotting module of the QPix420 system were also used. The same inoculation height for liquid to solid replication can also be used for solid to solid replicated source and target plates. The source plate contains a 12*8 matrix style E.coli clone (as shown in Figure 3B). The library of the source plate was inoculated into another OmniTray LB agar plate using the library replication module, and the target plate was clearly seen with 96 inoculated sample traces after inoculation. After overnight culture, 96 inoculated sample spots can clearly see good growth.
E.coli clone (Figure 4). This indicates that the QPix420 system can successfully transfer E. coli clones from the source OmniTray plate to the target OmniTray plate to achieve clonal replication of solid to solid plates.
Figure 4. Target OmniTray clone image after solid to solid agar plate replication. After the target OmniTray was cultured overnight at 37 degrees, 96 inoculated E. coli samples were able to grow normally, indicating that the QPix420 system can successfully replicate the cloned samples on the OmniTray LB plate to the target OmniTray LB agar plate.
The QPix400 series of microbial cloning screening systems enable fast, accurate liquid-to-solid, solid-to-solid automated sample replication operations through library replication modules and spotting head modules. 