(1) Select the appropriate fertilizer type. According to the agrochemical characteristics of the soil and the fertilizer requirements of the crop, suitable formula fertilizers should be selected. If the application is incompatible with the soil properties and the characteristics of the crops, it will not only cause waste of certain nutrients, but also lead to Reduce production. (2) Formula fertilizer is used in combination with simple fertilizer. The composition of the formula fertilizer is fixed, and it is difficult to meet the different requirements for nutrient elements in different soils, different crops, and different growth periods. The nutrient content of formula fertilizer should be used in conjunction with single fertilizer to ensure the coordinated supply of nutrients. (3) Choose the appropriate application method. Formula fertilizer should take appropriate technical measures in the application, in order to play a fertilizer effect; formula fertilizer as a base fertilizer, its effect is better than other single fertilizer. (4) To grasp the appropriate application period and the amount used. Formula fertilizer is a multi-element fertilizer, it is best to use basal fertilizer, try not to top dressing. According to soil strength, variety, yield to determine the appropriate application amount.
The RNA purification kit is used to purify and recover RNA molecules transcribed in vitro and total RNA extracted from various materials, which can effectively remove contaminating impurities in RNA samples. The recovery rate of this product can reach 80%, and the OD260/OD280 ratio of the obtained RNA is generally about 2.0, which can be directly used in subsequent sensitive experiments (such as microarray analysis, fluorescence RT-PCR, etc.). With the deepening of transcriptomics, the complexity of RNA types, expression regulation and functions is far beyond our imagination. Covid-19 Nucleic Acid Extraction Reagent,Nucleic Acid Extraction Reagent Kits,Dna Purification Kit,Rna Purification Kit Jilin Sinoscience Technology Co. LTD , https://www.jilinsinoscience.com
Removing ribosomal RNAs that account for more than 80% helps to focus sequencing on less abundant but informative RNAs. At present, the removal of rRNA is mainly through the combined use of probe and RNase H. The processed RNA will be mixed with many digestion products, enzymes and ions, which is not conducive to the subsequent construction of RNA library. Take the Columnar RNA Purification Kit as an example. Trizol is a ready-to-use reagent that can be used to purify total RNA from tissues and cells. This is a single-phase solution of phenol and guanidine isothiocyanate that facilitates lysis of tissues and cells, inhibiting RNases to maintain RNA integrity.