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First, mulberry, silkworm excrement pretreatment Spring silkworm rearing after mulberry summer cutting, cutting the branches in the field after drying into a semi-dry wood slicing machine and wood crusher for smash, due to strong phloem branches toughness, crushing machinery to make Appropriate changes, namely increase the strength of the sieve, the mesh diameter to 6 mm. The smashed wood chips are dried in the sun to prevent mold. The silkworm feces of 4-5 instar silkworms are mainly used to sift silkworm sand with a sieve, removing residual mulberry and new stems, and drying them for use.
Second, the ratio of cultivation materials The main formula: mulberry wood chips 80%, silkworm feces 18%. Sugar 1%, gypsum 1%; or mulberry wood chips 60%, cottonseed husk 20%, silkworm feces 19%, sugar 1%, gypsum 1%.
During the preparation, first soak the silkworm feces, make the silkworm feces absorb water, form a paste, add sugar and mix well, then stir with other materials and add water. The moisture content of the culture material should be pinched by hand, but not suitable for water flow.
Third, bagging, sterilization and inoculation with polyethylene tube for bacteria bags, specifications for 1350 cm or 1550 cm, thickness of 0.06 mm, about 80 bags per kilogram of film, about 0.7 kg per bag. When bagging, the materials in the bag are tight and uniform, and the ties are tight. Sterilization was carried out at normal pressure for 10-12 hours at 100°C. After the ceasefire, the temperature in the bag was reduced to below 30°C and the inoculation was taken out. Inoculate in a sterile or near-inoculated room, strains are attached to both ends of the bag, and the inoculation port is then taped.
Fourth, the bacteria bag material into the disinfected culture room, according to "well" shaped pile, 8-9 layer is appropriate. There is a channel between each row. Maintaining the indoor temperature of 20-25°C and relative humidity of 60%, the hyphae begin to move in 3-5 days and extend outward. From the 5th day, check once every 3-4 days to remove the bacterial rods contaminated by bacteria, or continue the culture after partial disinfection. After 25-30 days, the mycelium covered the entire bag.
5. When the bag is released, the interior of the bag is kept bright, and when the fruit body begins to grow, it can be taken out and moved to outdoor stocking. A plastic shed was set up to regulate the light and humidity in the stocking area. Relative humidity is maintained at 90%-95%. The mulberry garden can be used as a stocking place, and a breeding farm can also be established in the rice field. The fungus sticks can be placed on the shelf, or they can lean on bamboo sticks or mulberry branches.
6. Harvesting takes place at a temperature of 10-15°C and a relative humidity of 90%-95%, and the bags are stocked for about 25 days. The harvesting can be carried out for the first time. The tabs are opened, the edges are slightly curled, and slightly white ( Ear hairs) Mature plants with thin bases, cut off with a knife, rooted to avoid autolysis and rot or cause pests and diseases. After each harvest, appropriate ventilation, stop spraying 3-5 days, so that the wounds healed, and then depending on the growth of the harvest in stages.
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Techniques for cultivation of black fungus from mulberry silkworm silkworm excrement
Mulberry branches and silkworm feces are the main by-products of sericulture. The dry matter contained in the shoots contained 14.0% of crude protein. Total sugar 10.5%, crude fat 2.0%, iron 0.41%, potassium 0.45%; silkworm feces in the material contains crude protein 13.47%, crude fat 2.0%, whole sugar 19.4%, crude fiber 16.5%, nitrogen-free extract 57.5% Ash 9.9%. Both materials are good raw materials for growing black fungus. The use of mulberry wood chips and silkworm feces to cultivate black fungus can be harvested at least once compared to general culture materials. The related technologies are as follows: